Guide to Protein Purification (Methods in Enzymology, Vol. 182)
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Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.Key Features
* Detailed procedures newly written for this volume
* Extensive practical information
* Rationale and strategies for protein and enzyme purification
* Personal perspectives on enzyme purification by eminent researchers
Among the Topics Covered
* General methods for handling proteins and enzymes
* Extraction, subcellular fractionation, and solubilization procedures
* Comprehensive purification techniques
* Specialized purification procedures
* Protein characterization
* Immunological procedures
* Computer analysis of protein structure
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